nontumorigenic breast epithelial cell line mcf12a  (ATCC)

Journal: Pharmaceuticals

Article Title: Discovery and Evaluation of Novel Sulfonamide Derivatives Targeting Aromatase in ER+ Breast Cancer

doi: 10.3390/ph18081206

Figure Lengend Snippet: The percentage of non-tumorigenic MCF12A mammary cell viability with the most active compound 9 .

Article Snippet: Adenocarcinomic human breast epithelial cells (MCF7, ATCC HTB-22), the mouse fibroblast cell line (NIH3T3, ATCC CRL-1658), and the MCF12A human non-tumorigenic mammary epithelial cell line were purchased from ATCC (Manassas, VA, USA).

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: The neural stem cell gene PAFAH1B1 controls cell cycle progression, DNA integrity, and paclitaxel sensitivity of triple-negative breast cancer cells

doi: 10.1016/j.jbc.2025.110235

Figure Lengend Snippet: PAFAH1B1 silencing selectively reduces TNBC cell growth. A , TNBC cell lines MDA-MB-231, HCC38, and BT549 were transfected with either non-silencing control siRNA (siNS) or siRNA targeting PAFAH1B1 , and live cells were counted after 5 days using trypan blue exclusion or quantified using crystal violet staining. B , non-malignant breast cancer cell lines, MCF10A and MCF12A, were transfected and growth quantified after 5 days as in panel ( A ). Western blotting was performed at day 5 to confirm PAFAH1B1 silencing was maintained until the endpoint of the experiment. For all data, n = 3, points are technical replicates for each biological replicate, bars are means ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001 by unpaired two-tailed t test.

Article Snippet: MDA-MB-231, HCC38, BT549, MCF10A, and MCF12A cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Transfection, Control, Staining, Western Blot, Two Tailed Test